Table 1 of
Gross, Mol Vis 2000;
6:40-50.Table 1 of
Gross, Mol Vis 2000;
6:40-50.
Table 1. Mutagenesis primers
Primers listed are designated U for upper strand. The sequence for the mutant amino acid is in red. Primers for the lower strand are the reverse complements of the corresponding upper strand primers. Both an upper and lower strand primer must be used for each PCR reaction.
Amino acid
Mutant Primer Size Changed Primer sequence (5' to 3')
------ -------- ---- ---------- ----------------------------------
1 R1TSP1U 28 b V116N GCGGGTGGACAGCAACCCGGGCCAGGAG
2 R1TSP2U 32 b L147A CGCCTTAGTGCTGGATGCCCGGCACTGCACAG
3 R1TSP3U 34 b R148D CTTAGTGCTGGATCTCGATCACTGCACAGGAGGC
4 R1TSP4U 30 b G152A CCGGCACTGCACAGCGGGCCAGGTCTCTGG
5 R1TSP5U 27 b G153A CACTGCACAGGAGCCCAGGTCTCTGGC
6 R1TSP6U 28 b L208A GGATGTGGTGGTCGCCACCAGCAGCCAG
7 R1TSP7U 28 b E218A CAGGGGCGTGGCCGCGGACATCGCGCAC
8 R1TSP8U 27 b T237A GTGGGCGAGCGGGCGGGGGGAGGGGCC
9 R1TSP9U 29 b G239T CGAGCGGACTGGGACCGGGGCCGTGGACC
10 R1TSP10U 28 b I249A CCGGAAGCTGAGGGCGGGCGAGTCTGAC
11 R1TSP11U 34 b E251A GAAGCTGAGGATAGGCGCGTCTGACTTCTTCTTC
12 R1TSP12U 29 b G278A GTGGGAGGGCAGCGCGGTGCTGCCCTGTG
13 R1TSP13U 28 b P281A GCAGCGGGGTGCTGGCCTGTGTGGGGAC
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