Figure 1 of
Gross, Mol Vis 2000;
6:40-50.Figure 1 of
Gross, Mol Vis 2000;
6:40-50.
Figure 1. Sequence of IRBP Repeat 1
Mutant amino acids are just above the wild type amino acids being mutated. Primers are in red with names below the sequence. The wild type codons changed by site directed mutagenesis are in blue.
ATG GTA CCG AGC TCG GAT CCG GGC CCC ACA CAC CTG TTC CAG CCA AGC CTG GTG CTG
M V P S S D P G P T H L F Q P S L V L
GAC ATG GCC AAG GTC CTC TTG GAT AAC TAC TGC TTC CCG GAG AAC CTG CTG GGC ATG
D M A K V L L D N Y C F P E N L L G M
CAG GAA GCC ATC CAG CAG GCC ATC AAG AGC CAT GAG ATT CTG AGC ATC TCA GAC CCG
Q E A I Q Q A I K S H E I L S I S D P
CAG ACG CTG GCC AGT GTG CTG ACA GCC GGG GTG CAG AGC TCC CTG AAC GAT CCT CGC
Q T L A S V L T A G V Q S S L N D P R
CTG GTC ATC TCC TAT GAG CCC AGC ACC CCC GAG CCT CCC CCA CAA GTC CCA GCA CTC
L V I S Y E P S T P E P P P Q V P A L
ACC AGC CTC TCA GAA GAG GAA CTG CTT GCC TGG CTG CAA AGG GGC CTC CGC CAT GAG
T S L S E E E L L A W L Q R G L R H E
LexR1-1>
N
GTT CTG GAG GGT AAT GTG GGC TAC CTG CGG GTG GAC AGC GTC CCG GGC CAG GAG GTG
V L E G N V G Y L R V D S V P G Q E V
1
CTG AGC ATG ATG GGG GAG TTC CTG GTG GCC CAC GTG TGG GGG AAT CTC ATG GGC ACC
L S M M G E F L V A H V W G N L M G T
A D A A
TCC GCC TTA GTG CTG GAT CTC CGG CAC TGC ACA GGA GGC CAG GTC TCT GGC ATT CCC
S A L V L D L R H C T G G Q V S G I P
2 3 4 5
TAC ATC ATC TCC TAC CTG CAC CCA GGG AAC ACC ATC CTG CAC GTG GAC ACT ATC TAC
Y I I S Y L H P G N T I L H V D T I Y
AAC CGC CCC TCC AAC ACC ACC ACG GAG ATC TGG ACC TTG CCC CAG GTC CTG GGA GAA
N R P S N T T T E I W T L P Q V L G E
LexR1-2>
A
AGG TAC GGT GCC GAC AAG GAT GTG GTG GTC CTC ACC AGC AGC CAG ACC AGG GGC GTG
R Y G A D K D V V V L T S S Q T R G V
6
A
GCC GAG GAC ATC GCG CAC ATC CTT AAG CAG ATG CGC AGG GCC ATC GTG GTG GGC GAG
A E D I A H I L K Q M R R A I V V G E
7
A T A A
CGG ACT GGG GGA GGG GCC CTG GAC CTC CGG AAG CTG AGG ATA GGC GAG TCT GAC TTC
R T G G G A L D L R K L R I G E S D F
8 9 10 11
TTC TTC ACG GTG CCC GTG TCC AGG TCC CTG GGG CCC CTT GGT GGA GGC AGC CAG ACG
F F T V P V S R S L G P L G G G S Q T
LexR1-3>
A A
TGG GAG GGC AGC GGG GTG CTG CCC TGT GTG GGG ACT CCG GCC GAG CAG GCC CTG GAG
W E G S G V L P C V G T P A E Q A L E
12 13
AAA GCC CTG GCC ATC CTC ACT CTG CGC CAC CAC CAC CAC CAC CAC TGA TAA GGA TCC
K A L A I L T L R H H H H H H stop
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