Figure 1. Effect of laminin and cAMP on the morphological differentiation of WERI-Rb1 cells

A: Control cells attached on poly-D-lysine. B: Cells treated with laminin (12.5 mg/ml), forskolin (10 mM), and IBMX (0.5 mM). C: Cells treated with laminin alone. D: Cells treated with laminin/forskolin/IBMX and H89 (10 mM). E: Cells attached on poly-D-lysine and treated with forskolin/IBMX. Cells in the windows of B and C are those during early differentiation (day 5). Cells in B and C are those observed at the end of study (day 12). F: Percentage of morphologically changed cells in various treated groups. LN, laminin alone; LN/F/I, laminin/forskolin/IBMX; LN/F/I/H, laminin/forskolin/IBMX/H89. Shown are means±S.D. for each treatment group, N=9 per group. Asterisks indicate level of statistical significance: ^* for p<0.05, ^** for p<0.01. Magnification for panels A-E: 800x.