Figure 6 of
Yang, Mol Vis 2000;
Figure 6. Uptake and incorporation of [3H]all-trans-retinol into the chromophore of RGR
Normal (A) and Lentivirus-transduced (B) ARPE-19 cells were preincubated overnight in serum-free RPMI1640 medium and then incubated for 3 h with [3H]all-trans-retinol (10 mCi, 50 Ci/mmol) in RPMI1640. Total membrane proteins (~15 mg per lane) were prepared and analyzed by gel electrophoresis and fluorography. The autoradiographic film was exposed for 10 days.