Figure 5 of Yang, Mol Vis 2000; 6:237-242.

Figure 5. Binding of [3H]all-trans-retinal to recombinant human RGR opsin

Bovine RPE microsomal (A) and total cell membranes from nontransduced (B) and transduced (C) ARPE-19 cells were incubated with [3H]all-trans-retinal. The reaction products were separated by SDS-PAGE and analyzed by fluorography with 1-week exposure to autoradiographic film. Native bovine RGR and recombinant human RGR were capable of binding to [3H]all-trans-retinal in a specific manner in vitro.

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