Figure 5 of
Yang, Mol Vis 2000;
Figure 5. Binding of [3H]all-trans-retinal to recombinant human RGR opsin
Bovine RPE microsomal (A) and total cell membranes from nontransduced (B) and transduced (C) ARPE-19 cells were incubated with [3H]all-trans-retinal. The reaction products were separated by SDS-PAGE and analyzed by fluorography with 1-week exposure to autoradiographic film. Native bovine RGR and recombinant human RGR were capable of binding to [3H]all-trans-retinal in a specific manner in vitro.