Figure 2 of
Yang, Mol Vis 2000;
Figure 2. Expression of human RGR protein in COS-7 and ARPE-19 cells
(A) COS-7 and ARPE-19 cells were transfected with pcDNA3-hRGR (2 mg DNA/1 x 106 cells) using the Lipofectamine Plus agent (Life Technologies), according to the manufacturer's instructions. Alternatively, confluent cells were transduced with the pHR'-CMV-hRGR recombinant Lentivirus at equal transduction units (TU)/ml. Control cells were subjected to mock transfection or incubated without the Lentivirus. Each lane was loaded with 2 mg of total membrane protein. (B) In this experiment, the transduction frequency of the COS-7 and ARPE-19 cells was 10% and 30%, respectively. The transduction frequency was scored by immunohistochemical staining of transduced cells, and Western blot assays were performed with the DE7 antibody.