Figure 1. Time dependent accumulation of z-crystallins in E. coli cells

Time dependent accumulation of mutant (A) and wild-type (B) z-crystallins into the soluble and insoluble fractions of E. coli cells. Aliquots were drawn 1, 2, and 3 h after induction and the total cell lysate (T), soluble (S) and insoluble (I) fractions run on SDS-polyacrlamide gels. Molecular weight markers are indicated. The positions of z-crystallins and mutant z-crystallins are marked by arrows.