Figure 1 of
Choi, Mol Vis 2000;
Figure 1. Schematic representation of the GFP/18 kDa FGF-2 fusion protein
As described in the Methods section, a 471 bp ApaI-KpnI fragment, which retained the potential to generate the 18-kDaFGF-2 using the methionine (ATG) initiation site, was cloned into the multicloning site of pEGFP-C3. In parallel, the DNA fragment with the reversed sequence of 18 kDa FGF-2 was cloned and designated as pEGFP-18-kDa FGF-2/R.