Figure 8 of
Liang, Mol Vis 2000;
6:10-14.
Figure 8. ThT fluorescence with high molecular weight crystallin aggregates
ThT fluorescence was observed to increase with HMW aggregation for aA- and aB-crystallin. Protein samples were preheated at 62 °C for 2 h and then cooled to room temperature before fluorescence measurements were taken. Separate measurements were taken with no heating (0 h) and after two hours of heating at 62 °C (2 h).