Figure 7 of Liang, Mol Vis 2000; 6:10-14.

Figure 7. Bis-ANS fluorescence with high molecular weight crystallin aggregates

Bis-ANS fluorescence was observed to increase with HMW aggregation for aA- and aB-crystallin. Protein samples were preheated at 62 °C for 2 h and then cooled to room temperature before fluorescence measurements were taken. Separate measurements were taken with no heating (0 h) and after two hours of heating at 62 °C (2 h).

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