Figure 4 of
Guo, Mol Vis 2000;
Figure 4. HPLC analysis of deacylated [32P]PIP3 from anti-PY immunoprecipitates of PY-ROS
The immunoprecipitates were assayed for PI 3-kinase activity with [g-32P]ATP using PI-4,5-P2 as a substrate. The [32P]-labeled phospholipids were separated on TLC, deacylated, and analyzed by HPLC with flow-through scintillation counting. Dotted lines represent the elution profile of the standards gPI-4,5-P2 and I-1,3,4,5-P4.