Figure 4 of Guo, Mol Vis 2000; 6:216-21.

Figure 4. HPLC analysis of deacylated [32P]PIP3 from anti-PY immunoprecipitates of PY-ROS

The immunoprecipitates were assayed for PI 3-kinase activity with [g-32P]ATP using PI-4,5-P2 as a substrate. The [32P]-labeled phospholipids were separated on TLC, deacylated, and analyzed by HPLC with flow-through scintillation counting. Dotted lines represent the elution profile of the standards gPI-4,5-P2 and I-1,3,4,5-P4.

(7 K)

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