Table 2 of
John, Mol Vis 2000;
6:204-215.Table 2 of
John, Mol Vis 2000;
6:204-215.
Table 2. Cell-specific markers used in study
Cell specific antibodies were used to assess cone immunoreactivity in retinas with RHO mutations.
Cell Marker Specificity Species Dilution Source
------------------- --------- ------------------ ------- -------- ----------------
Cones 7G6 unknown mouse 1:250 P. MacLeish
Cones - X-arrestin mouse 1:100 G. Inana
Cones JH492 red/green rabbit 1:5,000 J. Nathans
cone opsin
Cones UW-16 red/green rabbit 1:200 J. Saari
cone opsin
Cones JH455 blue cone opsin rabbit 1:5,000 J. Nathans
Cones PNA- cone sheaths plant 1:100 Vector Labs
rhodamine lectin
(RL1072)
Interphotoreceptor - interphotoreceptor rabbit 1:100 J. Saari
matrix retinoid-binding
protein
Cones and rods Per peripherin-2 mouse 1:30 R. Molday
3Bg/5H2
Cones, rods P26 recoverin rabbit 1:1000 A. Dizhoor
bipolar cells
Cones, C8666 calbindin mouse 1:200 Sigma
Horizontal, bipolar
and amacrine cells
Cones, rods, A6403 cytochrome C mouse 1:1000 Molecular Probes
inner retinal cells oxidase
Rods 4D2 rhodopsin mouse 1:40 R. Molday
Rods - rhodopsin rabbit 1:3000 E. Kean
Rods A9C6 rod arrestin mouse 1:1000 L. Donoso
(S-antigen)
Astrocytes and ZO334 GFAP rabbit 1:750 Dako
reactive
Müller cells
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