Table 1 of
Simpson, Mol Vis 2000;
6:178-183.Table 1 of
Simpson, Mol Vis 2000;
6:178-183.
Table 1. Oligonucleotide primer sequences and rapid amplification conditions
The length of the specific amplification products and their approximate Tm are indicated (these varied ±0.3 °C depending on the template). *Primers may be suitable for use with murine (M) or human sequences (H). Accession numbers are given for the gene sequences from which primers are derived (certain sequences contain mismatches with the primers but have been shown to be amplified effectively). The asterisk ("*") marks an alternatively spliced product.
Gene M/H* Primer sequences(5'-3') MgCl2 Anneal Product
(Accession) Forward and Reverse (mM) temp bp & Tm
------------------ ----------------------- ----- ------ ---------------
28S rRNA (M/H) TTGAAAATCCGGGGGAGAG 4 54 100
X00525, M11167 ACATTGTTCCAACATGCCAG 87.1(M)86.9(H)
AGE-R3 (MAC-2) (H) GTTGCCTTCCACTTTAACCC 4 54 181
M35368 CATTCACTGCAACCTTGAAG 81.3(H)
ARP (36B4) (M/H) CGACCTGGAAGTCCAACTAC 4 53 109
X15267, M17885 ATCTGCTGCATCTGCTTG 82.4(M)80.5(H)
Cathepsin-D (M) ACAACAGTGACAAGTCCAG 3 55 321
X53337 TGTTCAGGTAGAAGGAGAAG 91.2(M)
Caveolin (M/H) TACAAGCCCAACAACAAG 5 55 209
U07645/Z18951 ACAGTGAAGGTGGTGAAG 87.1(H)
Cyclin D2 (M) CTTCCAAGCTGAAAGAGACC 4 53 111
M83749 TACCCAACACTACCAGTTCC 85.5(M)
Cyclophilin (H) CTCCTTTGAGCTGTTTGCAG 4 55 325
Y00052 CACCACATGCTTGCCATCC 86.0(H)
GAPDH (M) AACGACCCCTTCATTGAC 4 57 191
M32599 TCCACGACATACTCAGCAC 86.6(M)
HIF (M/H) ACAAGTCACCACAGGACAG 3 52 168
AF003695/U22431 AGGGAGAAAATCAAGTCG 83.1(M)
Laminin B1 (H) ACATTGACACGACAGACCC 4 56 231
NM_002291/M61951 GAAGACACAAGCACTGACC 88.2(H)
LR67 (M/H) TCACTAACCAGATCCAAGC 4 55 350
J02870, X15005 CTTGGTCACAGCCTTCTC 88.9(M)83.1(H)
MAC 1 (M) GATGAGACAAAGAACAACACAC 4 55 208
X07640 TGAAGAACCTCTGAGCATCC 86.2(M)
NRL (M) AGTCCCTTGGCTATGGAATATG 4 54 147
L14935 ACTGAAGGTGGGTGAAGGAG 87.5(M)
PDGFA (M/H) GTCCAGGTGAGGTTAGAGG 4 57 210/273*
M29464, X03795 CACGGAGGAGAACAAAGAC 88.8(M)
PDGFB (M/H) AAGCACACGCATGACAAG 5 55 109
X02811 GGGGCAATACAGCAAATAC 87.5(M)
PlGF (M) ACAGAAGTGGAAGTGGTG 4 56 503
X80171 GGCTAATAAATAGAGGGTAGG 87.2(M)
Rhodopsin (M) ACACCACTCAACTACATCC 5 57 463
M55171 (U22180) CGTAGCAGAAGAAGATGAC 90.3(M)
VEGF (M) TTACTGCTGTACCTCCACC 4 55 189
S38083 ACAGGACGGCTTGAAGATG 87.2(M)
VEGF (H) CGAAACCATGAACTTTCTGC 4 55 302
M32977 CCTCAGTGGGCACACACTCC 88.5(H)
VEGFD (M) ACCTCCTACATCTCCAAAC 3 56 422
D89628 TCCAGACTTTCTTTGCAC 86.0(M)
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