Figure 1. Elution profile of synthetic peptide standards

A linear gradient of 30-40% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid was used, over a period of 60 min, at a flow rate of 1.0 ml/min. Only the time interval containing the peptides of interest is shown. Solid arrow designates 28.2 pmoles of Peak 1, the purified amidated form of sequence 131-145 from human gS-crystallin. Open arrow designates 28.2 pmoles of Peak 2, the purified deamidated form of the same sequence.