Figure 3 of
Seigel, Mol Vis 2000;
Figure 3. IGF-1 and LongR3 enhance viability of hypoxic primary retinal cells
Postnatal day 7 rat retinal cell cultures were pre-treated with native IGF-1 (Panel A) or LongR3 IGF-1 analog (Panel B). Equivalent concentrations of IGF-1 or Long R3 were used (0, 10, 50, or 100 ng/ml). Primary retinal cells remained in pre-treatment medium and then subjected to hypoxia (95% N2/5% CO2) for up to 4 h. Cell survival was determined by trypan blue viability testing and cell counts at the 4 h endpoint. Error bars indicate standard deviations. IGF-1 or LongR3 showed statistically significant inhibition of cell death (p<0.05). There was no significant difference between 50 ng/ml and 100 ng/ml of either IGF-1 or LR3.