Figure 1 of
Seigel, Mol Vis 2000;
Figure 1. Native IGF-1 inhibits cell death of hypoxic R28 retinal cells
R28 cells were pre-treated for 24 h with varying concentrations of IGF-1 (0, 10, 50, and 100 ng/ml). R28 cells remained in IGF-1-containing medium and then subjected to hypoxia (95% N2/5% CO2) for up to 8 h. Cell survival was determined by trypan blue viability testing and cell counts at 2 h intervals. Error bars indicate standard deviations. There were significant differences between 10 ng/ml and 50 ng/ml at all time points (p<0.0065). There was no statistically significant difference between 50 ng/ml and 100 ng/ml at 2, 4, or 8 h.