Figure 2 of
Kuehn, Mol Vis 2000;
6:148-156.Figure 2 of
Kuehn, Mol Vis 2000;
6:148-156.
Figure 2. cDNA sequence of mouse IPM 150 and translation of the open reading frame
The C1 (amino terminal) and C2 (carboxy terminal) conserved domains are in blue. The EGF-like domain is indicated by red and the amino-terminal cysteine residues are marked by brown. Potential glycosaminoglycan attachment sites shaded green, N-linked glycosylation consensus sites are pink. Note that a CT dinucleotide repeat region occurs between nucleotides 3,586 and 3,636. This sequence is available under AF266478.
20 40 60 80 100
ACAAGATTATTCCAGGAACTGACCTGCTTCCGGATCCTCGAGAATTAGCACCTTCATAGGGTAAACCAGGGAGGTCGTCTGTACTCAGCCGGCACCTGGA
120 140 160 180
TTTGATTATTTTTCATATTTCAGTCACCTTATTTCTTTAAGTGTGACTTGGTATTGTTCTGTGATTTTTCAGAATTACCAGTATACAGAACCAGA ATG
Met
200 220 240 260
AAT TTT CAA ATT AAA CAT GCT ATC TTT GTT TTT GGG ATT TTT CTC CAA GTT CAA GGA ATC AAA GAT ACC TCT ATT
Asn Phe Gln Ile Lys His Ala Ile Phe Val Phe Gly Ile Phe Leu Gln Val Gln Gly Ile Lys Asp Thr Ser Ile
280 300 320 340
AAA ATA TTC AGT TCT GAA ATT AAA AAC ATA GAC AAA ACC CCA AGA ATC GAA ACA ATT GAA AGT ACT TCA ACA GTG
Lys Ile Phe Ser Ser Glu Ile Lys Asn Ile Asp Lys Thr Pro Arg Ile Glu Thr Ile Glu Ser Thr Ser Thr Val
360 380 400 420
CAC AAA GTG TCA ACC ATG AAA CGA ATA TTC GAT TTG CCA AAG CTT CGA ACC AAA AGA TCA GCA CTT TTC CCA GCT
His Lys Val Ser Thr Met Lys Arg Ile Phe Asp Leu Pro Lys Leu Arg Thr Lys Arg Ser Ala Leu Phe Pro Ala
440 460 480
GCT AAC ATC TGT CCA CAG GAA TCC TTG AGA CAG ATT TTA GCA AGT CTT CAA GAA TAT TAT AGA CTG AGA GTA TGT
Ala Asn Ile Cys Pro Gln Glu Ser Leu Arg Gln Ile Leu Ala Ser Leu Gln Glu Tyr Tyr Arg Leu Arg Val Cys
500 520 540 560
CAA GAA GTC GTG TGG GAA GCA TAT CGT ATC TTT CTG GAC CGA ATT CCT GAC ACA GAG GAA TAT CAA GAC TGG GTC
Gln Glu Val Val Trp Glu Ala Tyr Arg Ile Phe Leu Asp Arg Ile Pro Asp Thr Glu Glu Tyr Gln Asp Trp Val
580 600 620 640
AGC CTC TGC CAG AAA GAA ACC TTC TGC CTC TTT GAC ATT GGG AAA AAC TTC AGC AAC TCC CAG GAG CAC CTA GAT
Ser Leu Cys Gln Lys Glu Thr Phe Cys Leu Phe Asp Ile Gly Lys Asn Phe Ser Asn Ser Gln Glu His Leu Asp
660 680 700 720
CTT CTT CAG CAG AGA ATA AAA CAG AGA AGC TTC CCT GGG AGG AAA GAT GAG ACA GCC TCC ATG GAG ACA CTG GAA
Leu Leu Gln Gln Arg Ile Lys Gln Arg Ser Phe Pro Gly Arg Lys Asp Glu Thr Ala Ser Met Glu Thr Leu Glu
740 760 780
GCA CCT ACT GAA GCC CCT GTG GTA CCC ACA GAT GTT TCC AGG ATG TCC CTG GGG CCC TTC CCA CTT CCT TCT GAT
Ala Pro Thr Glu Ala Pro Val Val Pro Thr Asp Val Ser Arg Met Ser Leu Gly Pro Phe Pro Leu Pro Ser Asp
800 820 840 860
GAC ACA GAC CTC AAG GAG ATT CTC AGT GTC ACC CTC AAG GAC ATT CAA AAG CCC ACA ACA GAA AGT AAA ACA GAA
Asp Thr Asp Leu Lys Glu Ile Leu Ser Val Thr Leu Lys Asp Ile Gln Lys Pro Thr Thr Glu Ser Lys Thr Glu
880 900 920 940
CCT ATT CAC GTG TCT GAA TTC TCA TCA GAG GAG AAG GTG GAA TTC AGC ATC TCT CTG CCA AAC CAC AGG TTC AAG
Pro Ile His Val Ser Glu Phe Ser Ser Glu Glu Lys Val Glu Phe Ser Ile Ser Leu Pro Asn His Arg Phe Lys
960 980 1000 1020
GCA GAG CTC ACC AAC TCT GGG TCA CCA TAC TAC CAG GAA CTG GTG GGA CAG TCC CAA CTG CAG TTG CAA AAG ATA
Ala Glu Leu Thr Asn Ser Gly Ser Pro Tyr Tyr Gln Glu Leu Val Gly Gln Ser Gln Leu Gln Leu Gln Lys Ile
1040 1060 1080
TTT AAG AAA CTT CCA GGA TTC GGA GAA ATC CGT GTA TTA GGA TTT AGA CCA AAG AAA GAA GAA GAT GGT TCA AGC
Phe Lys Lys Leu Pro Gly Phe Gly Glu Ile Arg Val Leu Gly Phe Arg Pro Lys Lys Glu Glu Asp Gly Ser Ser
1100 1120 1140 1160
TCC ACA GAA ATA CAG CTT ATG GCC ATC TTT AAG AGG GAC CAT GCA GAA GCA AAA AGC CCT GAT AGT CAT CTA CTG
Ser Thr Glu Ile Gln Leu Met Ala Ile Phe Lys Arg Asp His Ala Glu Ala Lys Ser Pro Asp Ser His Leu Leu
1180 1200 1220 1240
TCT CTT GAT TCC AAC AAA ATT GAA AGT GAA AGA ATC CAT CAT GGA GTC ATA GAA GAC AAA CAA CCA GAA ACC TAC
Ser Leu Asp Ser Asn Lys Ile Glu Ser Glu Arg Ile His His Gly Val Ile Glu Asp Lys Gln Pro Glu Thr Tyr
1260 1280 1300 1320
CTC ACA GCT ACA GAC CTC AAA AAA CTC ATC ATA CAA CTA CTA GAT GGA GAC CTG TCC TTG GTA GAA GGG AAA ATT
Leu Thr Ala Thr Asp Leu Lys Lys Leu Ile Ile Gln Leu Leu Asp Gly Asp Leu Ser Leu Val Glu Gly Lys Ile
1340 1360 1380
CCA TTC GGT GAT GAA GTT ACT GGG ACA CTC TTC AGA CCT GTC ACT GAA CCA GAT CTG CCC AAG CCC CTT GCT GAT
Pro Phe Gly Asp Glu Val Thr Gly Thr Leu Phe Arg Pro Val Thr Glu Pro Asp Leu Pro Lys Pro Leu Ala Asp
1400 1420 1440 1460
GTC ACA GAG GAT GCC ACT TTG AGT CCA GAA CTT CCT TTC GTT GAG CCT AGG CTT GAG GCA GTG GAC AGA GAA GGA
Val Thr Glu Asp Ala Thr Leu Ser Pro Glu Leu Pro Phe Val Glu Pro Arg Leu Glu Ala Val Asp Arg Glu Gly
1480 1500 1520 1540
TCT GAG CTG CCT GGA ATG TCC TCC AAA GAC AGT TCT TGG TCT CCA CCT GTA TCA GCC TCA ATT TCC CGA TCA GAA
Ser Glu Leu Pro Gly Met Ser Ser Lys Asp Ser Ser Trp Ser Pro Pro Val Ser Ala Ser Ile Ser Arg Ser Glu
1560 1580 1600 1620
AAT CTA CCT TCG TTT ACA CCT AGC ATC TTC TCT CTA GAT GCT CAA AGC CCC CCT CCC TTG ATG ACC ACT GGC CCA
Asn Leu Pro Ser Phe Thr Pro Ser Ile Phe Ser Leu Asp Ala Gln Ser Pro Pro Pro Leu Met Thr Thr Gly Pro
1640 1660 1680
ACA GCA CTC ATC CCC AAG CCC ACT CTC CCC ACT ATC GAT TAT TCT ACC ATC CGC CAA TTG CCT CTG GAA TCG TCA
Thr Ala Leu Ile Pro Lys Pro Thr Leu Pro Thr Ile Asp Tyr Ser Thr Ile Arg Gln Leu Pro Leu Glu Ser Ser
1700 1720 1740 1760
CAT TGG CCT GCA TCC TCC AGT GAC AGA GAG CTG ATC ACA AGC AGC CAT GAC ACA ATC CGA GAC CTA GAT GGC ATG
His Trp Pro Ala Ser Ser Ser Asp Arg Glu Leu Ile Thr Ser Ser His Asp Thr Ile Arg Asp Leu Asp Gly Met
1780 1800 1820 1840
GAT GTG TCT GAC ACG CCA GCC TTG TCA GAA ATA TCA GAA CTG AGT GGA TAC GAT TCT GCC TCG GGT CAG TTC TTG
Asp Val Ser Asp Thr Pro Ala Leu Ser Glu Ile Ser Glu Leu Ser Gly Tyr Asp Ser Ala Ser Gly Gln Phe Leu
1860 1880 1900 1920
GAG ATG ACC ACA CCC ATC CCA ACA GTA CGG TTC ATC ACC ACC AGC TCC GAG ACC ATT GCC ACC AAG GGC CAG GAG
Glu Met Thr Thr Pro Ile Pro Thr Val Arg Phe Ile Thr Thr Ser Ser Glu Thr Ile Ala Thr Lys Gly Gln Glu
1940 1960 1980
CTA GTG GTA TTC TTC AGC CTG CGT GTT GCT AAC ATG CCG TTC TCC TAT GAC CTG TTC AAC AAG AGT TCT CTG GAG
Leu Val Val Phe Phe Ser Leu Arg Val Ala Asn Met Pro Phe Ser Tyr Asp Leu Phe Asn Lys Ser Ser Leu Glu
2000 2020 2040 2060
TAT CAA GCC CTG GAA CAA CGA TTC ACA GAC CTG CTG GTT CCC TAT CTA CGA TCG AAT CTT ACG GGA TTT AAG CAA
Tyr Gln Ala Leu Glu Gln Arg Phe Thr Asp Leu Leu Val Pro Tyr Leu Arg Ser Asn Leu Thr Gly Phe Lys Gln
2080 2100 2120 2140
CTG GAA ATA CTC AGC TTC AGA AAC GGA AGT GTG ATC GTG AAC AGC AAA GTG CGG TTT GCA AAG GCG GTA CCC TAC
Leu Glu Ile Leu Ser Phe Arg Asn Gly Ser Val Ile Val Asn Ser Lys Val Arg Phe Ala Lys Ala Val Pro Tyr
2160 2180 2200 2220
AAC CTC ACC CAG GCC GTG CGC GGG GTC TTG GAG GAT CTT CGG TCC ACC GCA GCT CAA GGG CTC AAT CTG GAA ATC
Asn Leu Thr Gln Ala Val Arg Gly Val Leu Glu Asp Leu Arg Ser Thr Ala Ala Gln Gly Leu Asn Leu Glu Ile
2240 2260 2280
GAA AGC TAC TCC CTC GAC ATT GAA CCA GCT GAT CAG GCG GAT CCC TGC AAA CTC CTA GAC TGT GGC AAA TTT GCC
Glu Ser Tyr Ser Leu Asp Ile Glu Pro Ala Asp Gln Ala Asp Pro Cys Lys Leu Leu Asp Cys Gly Lys Phe Ala
2300 2320 2340 2360
CAG TGT GTA AAG AAT GAG TGG ACA GAG GAA GCA GAG TGT CGC TGC AGA CAG GGA CAT GAG AGC CAC GGG ACC CTG
Gln Cys Val Lys Asn Glu Trp Thr Glu Glu Ala Glu Cys Arg Cys Arg Gln Gly His Glu Ser His Gly Thr Leu
2380 2400 2420 2440
GAC TAC CAG ACC CTG AAC CTC TGT CCC CCT GGA AAG ACT TGT GTG GCC GGC CGA GAA CAA GCA ACT CCA TGC AGG
Asp Tyr Gln Thr Leu Asn Leu Cys Pro Pro Gly Lys Thr Cys Val Ala Gly Arg Glu Gln Ala Thr Pro Cys Arg
2460 2480 2500 2520
CCA CCA GAT CAC TCT ACA AAC CAA GCT CAG GAA CCT GGT GTT AAA AAG CTA CGT CAG CAA AAT AAG GTA GTC AAG
Pro Pro Asp His Ser Thr Asn Gln Ala Gln Glu Pro Gly Val Lys Lys Leu Arg Gln Gln Asn Lys Val Val Lys
2540 2560 2580 2600
AAA AGA AAT TCT AAA CTA TCA GCT ATA GGA TTT GAA GAA TTT GAA GAC CAG GAC TGG GAG GGA AAT TAAAAGCTGGA
Lys Arg Asn Ser Lys Leu Ser Ala Ile Gly Phe Glu Glu Phe Glu Asp Gln Asp Trp Glu Gly Asn STOP
2620 2640 2660 2680 2700
ATCATATGCATTATGTTGCAAACTCTGTTGAAAGGAAACTTTATTTCTTAAAGAAAGGTGTATCTGTTCTGTTAACTTCTGAAAAACAGAGGGAGAGATT
2720 2740 2760 2780 2800
CAGTGGTCATTGGAATACAGGCATGTAATCAACTTTGAGACTCAGCATGCTTGAACAAGAGCACAGGCGTGTATTTGATGACAGTTAAGCCTGGTTGGGG
2820 2840 2860 2880 2900
CGGGGGGCACATATTTTTAGTCAAAACTCAAAGCAATCATTGGAACACATTTGACTATTTTTGGACAGTACTCAAGTAGCAAAGATAAGGTTAGCTTTTT
2920 2940 2960 2980 3000
TCTTTCTTTAAATTATTACATAAARCTTATTTCAAATAAATACAACTTGTTTAGTGGGTTGTACAATATTGAGGATCTGATTCTTTTATATGTTAGAATA
3020 3040 3060 3080 3100
TACAGTTAAAAGATTATCATTTGGGCCAGAGAGATAGCTAAGTGGTTAAGAGTATATACTGCTCTTCCAGAAGCCCTGGGTTTACCGTCCCAACAGCCAC
3120 3140 3160 3180 3200
ATTGACTGGCTCACACACACCTGTAAGTCAGGCTCCAGAGAACAAACACCCTCCTCTGGCCTTTGTACCCACGTGCACATAACCGCAAACAGACACACCC
3220 3240 3260 3280 3300
ACGCTATTTTTTTAGAAGTCATTGATTTTTTTAATTAGGGGTGGAAAAKCAGGCTGGAGAGATGACTCCGTGGTTAAGAACAGTTGTTGTTCTTCCAGAG
3320 3340 3360 3380 3400
GACCCAGGTTCAGTTCCCAGAACCCACAAGGCNAGTCTCCCAACTATTCATAATTCTAGTTCAAGTGGATCCAGCACCCTCTTCTAACTGATACTGCCAG
3420 3440 3460 3480 3500
TACCAGGCAGCCATGTGGTGCATATGCATTTGGGCAGGTAAAACACTCAGACACGCAAAAAATTTTAAATCTAAATTTTGAAAATATTTTAGTTTTAAGG
3520 3540 3560 3580 3600
ATGATCACTGTGTGAGGGTCAGGTCTCTTATGTATGAATGTAGTACCAAGAACTGTGATGAGTATATGTATGCTCCATTCTATAGTCTCCTCTCTCTCTC
3620 3640 3660
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGGAATTCCGGAATTCCGGAATTCCGGAATTCCG
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