Figure 8 of
Cai, Mol Vis 2000;
Figure 8. FRI of vinculin and actin in HTM cells treated with LAT-A
Panels A-L: Digital microscopic analysis of HTM cells, double-labeled for vinculin (VIN; CY3) and actin (AC; FITC), before (0) and after 15 and 60 min treatment (15', 60') with 1 mM LAT-A. Panels M-T: Cells treated with LAT-A for 1 h and then incubated in medium without LAT-A for 5 or 20 min (60'+5'R; 60'+20'R). The left column shows the superimposed images of vinculin (red) and actin (green). The columns marked CY3 and FITC show the intensity of labeling of the respective proteins, using the spectrum scale presented under the FITC column. The FRI column depicts the CY3-to-FITC intensity ratio (scale shown under the column). Bar = 10 mm.