Figure 10 of
Cai, Mol Vis 2000;
Figure 10. FRI of paxillin and phosphotyrosine in HTM cells treated with LAT-A
Panels A-L: Digital microscopic analysis of HTM cells, double-labeled for paxillin (PAX; CY3) and phosphotyrosine (PT; FITC), before (0) and after 15 and 60 min treatment (15', 60') with 1 mM LAT-A. Panels M-T: cells treated with LAT-A for 1 h and then incubated in medium without LAT-A for 5 or 20 min (60'+5'R; 60'+20'R). The left column shows the superimposed images of paxillin (red) and phosphotyrosine (green). The columns marked CY3 and FITC show the intensity of the respective labeling, using the spectrum scale presented under the FITC column. The FRI column depicts the CY3-to-FITC intensity ratio (scale shown under the column). Bar = 10 mm.