Figure 1. Two-dimensional model and amino acid sequence of rat rhodopsin

The first 39 amino acids (dashed green circles, green and black letters) were not sequenced with MS/MS. The blue dashed circles and blue letters for the last 19 amino acids denote the fragment cleaved by Asp-N. The detected posttranslational modifications are also indicated as well as the site of the P23H mutation (black letter). The posttranslational modifications are identical with those in bovine rhodopsin. Notice that amino acid 320 is a threonine and not a serine (black letter). Phosphorylation sites are shown in black. The numbers indicate the beginning of the corresponding CNBr fragment.