Figure 2 of Steele, Mol Vis 2000; 6:85-94.

Figure 2. Strategy for engineering To3 into the 129/SVJ mouse Lim2 gene

The 5' end of the gene was amplified via PCR from To3/To3 genomic DNA. This fragment was then ligated to the indicated 3' end fragment, which was purified from the original isolated 129/SvJ genomic clone. Asterisk (*) above exon 2 indicates To3 mutation.

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