Figure 2 of
Steele, Mol Vis 2000;
Figure 2. Strategy for engineering To3 into the 129/SVJ mouse Lim2 gene
The 5' end of the gene was amplified via PCR from To3/To3 genomic DNA. This fragment was then ligated to the indicated 3' end fragment, which was purified from the original isolated 129/SvJ genomic clone. Asterisk (*) above exon 2 indicates To3 mutation.