Figure 4 of
Wistow, Mol Vis 2000;
6:79-84.Figure 4 of
Wistow, Mol Vis 2000;
6:79-84.
Figure 4. The 3'- end of the CRYGS gene
Exons 2 and 3 are indicated, with coding sequences marked in red. The alternative polyadenylation signals are highlighted in blue. Sequences from Hs.134126, the Mys sequences, are in pink. The A11 stretch that may be responsible for cDNA priming of these sequences is shown in orange. The cryptic exon (CE) spliced into a gS cDNA is in green with flanking splice junctions in bold type. An additional single EST not included in the Unigene cluster but which may also be part of the Mys sequence is shown in brown. All the sequences shown here were experimentally determined independently in this study.
ATGAGTCATTCTTCGTTTCTTCCTCTCATTCCCTCCATCTAATTAGTCACCAAATCCTGAAGTTCTTTACTGTTTTCTCA
TCATCATTCACATTGGAGTTGCCTTTTGGTTGGCCCTCATCATCTCTCACCTGGCCCACAGCAGCCATCCGTTTCTAGGC
ACACACTACTAAGTCCAACTCACTCATAATCACCAAAGGGATTCCAGCATGATTCTGGTGTCATTTTTCTGGCTTTATTT
TCTAGTACTTCACCACATATTCAATATGTACTGGACTTAATGAACTACTTATTGTCCCTATAATGGGCCATGTTCTTTCA Hs.134126
CTTTATTCTTTTAAACATGTTTGTTCCTTTTCATTTGCCTCTGGAAAACACCTACTTATCCTTCAATGCTCAGCTCAAAG
ATCCTCTCAAGACAGTATCCGGCTCTGCTCCTTTCATCTGAAATGACTCACATAGTTTTCGCCCGTGAAATCCATAAAGT CE
AAATCAGCCACAAGGTGGCGGCATAATGCCAGGTAATTAACGGTTCGAAGCATGACACCTGTGGTTTCAGTTTATAACCT
AGCATTGATCTGAAATTCCATCTTAAAAAAAAAAAGATGAAAAGATTGTGGAATTATTGAATAACAAGGGACAAGCTCTG
TAGCTCATTTGTAGTTTTCATCGTGGTTTTCAGGAACTGCTTGGTGAATCCTTGGGAGTTCCTAGGAACTCTGTCTCCTG INTRON 1
TTTCAGCCAGAAAAGCTCTGAGAGTTGTTTTAAAGCCAGAACTTCCAGTCTGCTGTAGTCTTCAAATAGGAAGCTGAAGC
TCAGAGAGGTGGCTGGATGAAGATAGTAAGGCTAATTGTAGAGAGGGAGCTCTGTAGTCCAGACTTACTGTTTCTGCTTT
CACAGGGCTGCATATTTTTCAGTGCCATCAGAGACTTACCCCAGGCATTGTGGTAGAAACTTGATAAGCGTTGGAGGCAA
AGAGGATTCTTATCATTTTGTTACTTTGCAACTACTCTGTTGGCAATAAACTAATAATAAAGAACATGATATGATACATT AI807504
TTCATGTATGAAAGCTCTACATCTATCTAAAATAAGCTCTGTAAAGCTAAGGTTCATGCTTTATTTTTCTCTATGTATCC
TCCATATCACCTAGGAGTCATCTTCATGAAACAATTATTGAAGTCAAGTGATGGGCACAGAGGATTGATAGCTCAAATAA
GGCTTGGTACTTGCCCTTGGAAAAAGTATGCTAGGATTCTGGTGGGAGGGGTAGACATATAAATGGATAATTTATAATAT
GATGTAAGTACAGAGAGCTGTGGGAACATAAAGGAAGGAAAATGAACTTGAGTCTGAGACTGCTCACTTCTATTAGAGCC
CTCTTCTGTTTTGCTTCATGTTCAGCCTTCAGGAAGAACTCATTTCTGTTGTTGACAACTGAAGTTGTCTGTCAGAAAGC
AATGTTGTAAGGTGACGTACAGCTACATATTTTTCCTCAAAATTGAGGTGAAAGGAATTTCTAAAGTAGGCATTATGTTC
I T F Y
TTAATTTTTATCTGTGAATTAAGCCACCCAGCTCCTCAGCTCTTTCTCTGTTGGCCCTCTACTTCAGATTACTTTCTATG
E D K N F Q G R R Y D C D C D C A D F H T Y L S R C N
AAGACAAAAATTTTCAAGGCCGTCGCTATGACTGTGATTGCGACTGTGCAGATTTCCACACATACCTAAGTCGCTGCAAC EXON 2
S I K V E G G T W A V Y E R P N F A G Y M Y I L P Q G
TCCATTAAAGTGGAAGGAGGCACCTGGGCTGTTTATGAAAGGCCCAACTTTGCTGGGTACATGTACATCTTACCACAGGG
E Y P E Y Q R W M G L N D R L S S C R A V H L
AGAGTACCCTGAATACCAGCGTTGGATGGGCCTCAACGACCGCCTCAGCTCCTGCAGAGCTGTTCATCTGGTGAGTCTGG
GGACTGTGTACTCCCTCTGTTTCCCACGCCCTCTGTCCGCTTTCTCTTGCTTTTAGGTGAACATTTtCTTGAGTCTTCTC
TACCTCTTAATGAGTAGCTGCTTGTTGGCTGCTGAGGCTTGAGTAACATCTTAGAGCAGCTCATCACTAGAATGACTACA INTRON 2
CTAATTTCCAGGGTGCTGTCTCTGCCTCTTGAGTGTTTTGAAGGATTAATTTTTTCTTATTGAAAGTTAGCTGTTTTAGT
CAGTACATGGTGAGATGGGAGTTCAGATCATCTAGCTTTCAAGGtGaTAAGTTCTGAGCAATACTGTTCTCTAAGTTCTT
P S G G Q Y K I
GACCTGCTGGTGATTTCCATAATGGTTTCTGTTCATTTTCTCCTGTGTCTTTTCAGCCTAGTGGAGGCCAGTATAAGATT
Q I F E K G D F S G Q M Y E T T E D C P S I M E Q F H
CAGATCTTTGAGAAAGGGGATTTTAGTGGTCAGATGTATGAAACCACCGAAGATTGCCCTTCCATCATGGAGCAATTTCA
M R E I H S C K V L E G V W I F Y E L P N Y R G R Q
CATGCGAGAGATCCACTCCTGTAAGGTGCTGGAGGGTGTCTGGATTTTCTATGAGCTACCCAACTACCGTGGCAGGCAGT EXON 3
Y L L D K K E Y R K P I D W G A A S P A V Q S F R R I
ACCTCCTGGACAAGAAGGAGTACCGGAAGCCCATCGATTGGGGTGCAGCCTCCCCAGCTGTCCAGTCTTTCCGCCGCATT
V E *
GTGGAGTAATGACATGAATGGGGCCATATTCTTCCTGGGGCCCAAATGCTGGCTGGCCTTGTGGTCCAAATAGGCATCAT
CAATAAAACAGTTGGCATGCATCCCACTGCTGACTATAATGCCTCTCCTTAAATGCTTCTAGGGACCAGCAATACAGTGC
TCGCCACAGTGGGCAGTCACACAAAGCTACCCATCTGCCAGATCACCAATCTAGATCTTTGTGCCAAACAAAATGAGATC
TCATTAAAAGGTTAGAAAGTCAA
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