Figure 1. Results of ELISA assays for determining the specificity of antibody to peptide 3R

A. ELISA with antiserum to peptide 3R. Microtiter wells were coated with aA-crystallin derived from aged human (open circle), 11-month-old human (open square), aged bovine (open triangle), and calf (open diamond) lenses. Blank wells were not coated with aA-crystallin (filled circle). Antiserum was diluted with 10 mg/ml BSA in PBS. B. ELISA with antibody purified with bovine aA-crystallin column. Microtiter wells were coated with peptide 3R (open circle) and aA-crystallin derived from aged human (open triangle) or aged bovine lenses (open square). Blank wells were not coated with antigen (filled circle). Original antibody concentration was A₂₈₀=0.084 and diluted with 10 mg/ml BSA in PBS. C. ELISA with antibody purified with peptide 3R column. Microtiter wells were coated with the same antigens as in B. Original antibody concentration was A₂₈₀=0.109 and diluted with 10 mg/ml BSA in PBS. D. ELISA with antibody purified with peptide 3R column. Microtiter wells were coated with peptide 3R (open circle), L-a-Asp T18 (open diamond), D-a-Asp T18 (X), L-b-Asp T18 (open square), and D-b-Asp T18 (open triangle), respectively. Blank wells were not coated with antigen (filled circle). Original antibody concentration was A₂₈₀=0.109 and diluted with 10 mg/ml BSA in PBS.