Figure 5. Expanded view of chromatographs and associated spectral data for Mitf^vit and C57BL/6 control mice at 10 weeks of age

The chromatographic data are taken from Figure 4A and Figure 4B. These data show an expanded view of the peaks having the same retention times as authentic 9-cis, 13-cis, and all-trans RA standards. Panel A (bottom) shows an expanded view of the chromatographic data for Mitf^vit at 10 weeks of age. Using an on-line photodiode array detector, spectral data were obtained for each of these peaks, which are shown in the top of panel A. Note that the absorbance maximum for the second peak is blue shifted in comparison to the first and third peaks. Panel B (bottom) is an expanded view of the chromatographic data for C57BL/6 control mice at 10 weeks of age. Spectral data associated with these peaks are shown in the top of panel B.