Table 1 of
Gonzalez-Fernandez, Mol Vis 1999;
5:41.Table 1 of
Gonzalez-Fernandez, Mol Vis 1999;
5:41.
Table 1. Oligonucleotides (5' to 3') used to amplify the 11-cis retinol dehydrogenase gene for SSCP and direct sequencing
Exon Sense Antisense
---- -------------------------- --------------------------
PCR primers for SSCP and autoradiographic sequencing
2 CCTGTAGGTCACTTGGGCTC CCCACACCATATTCCTGGTG
3 CAGCATCCTTTTCATCTCCC TGTTAGTCCTGGAACCCAGA
4 CAAGAACCCAGCAACTTCGC GTTTCCCTTCATGTGCCCCT
5 CTGATTGCAACCACCTATGG CAATCTCTTGCTGGAAGGCT
PCR primers for fluorescence sequencing*
2 TTTGCTTTGAACAGATGAGCC TGTGGGGAGATGAAAAGGATG
M13-GCCAATTATCTGCCAACCAG# M13-GCTTCTCCTCAAATTGTCCC**
3 TCCTGATCCCCACAGTCACC TGGCGACAGAGCAAGACTCC
M13-GCTAAAGGGACAATTTGAGGAG M13-GGTCACAAAGCTTACATAAGGG
4 ATACTTTCCTCCCTCTACCCC TTTCCCTTCCTCACTCCAGC
M13-AAAACTCTTGTCCCTGGTCTG M13-ACATCCTGTTCACCCTCAAC
5 TGAGGGTGAACAGGATGTTAC GCAACCTTACTCCCCTTCAG
M13-ATGTGGAACCTGCCCACTC M13-GTAGATGTGAGGCAGTTCCC
|
*The second primer pair for each exon region corresponds to the nested set.
#For sense M13 = TGTAAAACGACGGCCAGT (M13 forward sequence). The nested sense primer is biotinylated at the 5' end
**For antisense M13 = CAGGAAACAGCTATGACC (M13 reverse sequence).