Figure 1 of
Thulin, Mol Vis 1999;
**5**:40.

Figure 1. Phosducin (Pd) and PhLP inhibit G_{t} binding to
light-activated rhodopsin (Rho*)

Light-induced binding of ^{125}I-G_{t}[alpha] (0.2 µM) and
G_{t}ß[gamma] (0.2 µM) to urea-stripped rod outer segment
membranes (1.0 µM Rho) was carried out in the presence of Pd (green,
filled circles) or PhLP (red, open squares) at the concentrations
indicated (see Methods). Ovalbumin shown as negative control (blue, open
diamonds). Error bars represent the first standard deviation of data
from three separate experiments. 100% is 0.07-0.1 pmol of
^{125}I-G_{t}[alpha]bound/pmol of Rho*. Lines represent
non-linear least squares fit of the data to the equation: Fraction bound
= (1 - X)/(1 + [Pd]/K_{1/2}) + X, where X is the fraction remaining
bound at saturating the concentration of Pd and K_{1/2} is the
concentration of Pd at which half maximal inhibition occurred.
K_{1/2} values were 0.37 µM for Pd and 0.41 µM for
PhLP.

Thulin, Mol Vis 1999;

©1999 Molecular Vision <http://www.molvis.org/molvis/>

ISSN 1090-0535