Figure 3 of
Seigel, Mol Vis 1999;
Figure 3. Y79 cell suspension culture
Y79 retinoblastoma cells were obtained from the American Type Culture Collection (ATCC# HTB-18) and maintained in RPMI-1640 medium (Sigma, St. Louis, MO) with 15% fetal calf serum (heat-inactivated; Hyclone, Logan, UT), 0.02% L-glutamine and 50 µg/ml gentamicin. Cells were grown in suspension flasks in a 37 °C humidified chamber with 5% CO2, 95% air.
Note cells in "grape-like" clusters. Scale bar is 10 µm.