Figure 1. Cloning strategy for ligation of MIP26 into pSIN and thence into pVB6

Both MIP and the kanamycin resistance factor Neomycin phosphotransferase (NPTII) are flanked at their 3' end by Nopamine Synthase (NOS) flanking sequence [8], part of the wild-type A. tumefaciens Ti plasmid [8]. Genes appended with this sequence have been demonstrated to be transferred to plant tissue more efficiently [8]. Since MIP receives its own Cauliflower Mosaic Virus (CMV) promoter from the pSIN vector, it is does not matter in which direction it ligates into the final pVB6 vector.