Table 2 of
El-Kabbani, Mol Vis 1999;
5:20.Table 2 of
El-Kabbani, Mol Vis 1999;
5:20.
Table 2. Kinetic constants of wildtype and mutant ALR2
Kinetic constants are expressed as mean ± S.D. for 3 and 4 replicate experiments for determining Kd, and Km and kcat values, respectively. Km and kcat for NADPH were determined using 10 mM glyceraldehyde as substrate.
Substrate Wildtype P216S P216S/Wildtype DL-Glyceraldehyde Km (mM) 0.053 ± 0.008 0.161 ± 0.027 3.0 kcat (s-1) 1.19 ± 0.12 4.49 ± 0.48 3.8 kcat/Km (s-1 M-1) 22700 ± 3450 29000 ± 8320 1.3 NADPH Kd (nM) 7.4 ± 1.4 37.7 ± 6.4 5.1 Km (µM) 13.3 ± 1.8 19.7 ± 5.0 1.5 kcat (s-1) 0.9 ± 0.1 3.4 ± 0.8 3.8 kcat/Km (s-1 M-1) 65200 ± 5200 185000 ± 66200 2.8 NADP+ Kd (nM) 5.8 ± 0.7 31.3 ± 6.1 5.4 |