Figure 1 of
Takemoto, Mol Vis 5:2, 1999.
Figure 1. Preparation of [alpha]-A crystallin
Resolution of total proteins from nuclear sections of a 71 year old normal lens (top; 71N) and 65 year old cataractous lens (bottom; 65C) with opacification of the nucleus. See Methods for details of lens dissection, protein dissolution, and reduced carboxymethylation. Approximately 0.5 mg of protein was resolved on a C18 wide pore column (4.6 mm x 250 mm, Rainin Instruments), using a linear gradient of 30 - 80% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid, over a period of 30 min, at a flow rate of 1.0 ml/min. Horizontal bars designate the [alpha]-A crystallin peaks collected for further analysis.