Figure 5. Immunofluorescent localization of procollagen I and Hsp47 in bafilomycin A1-treated CEC

Cells were treated with 5 µg/ml bafilomycin A1 for 60 min, fixed, permeabilized, and stained as described in the text. A. Procollagen I (green) and Hsp47 (red) in bafilomycin A1-treated cells; B. Prolyl 4-hydroxylase (green) and Hsp47 (red) in bafilomycin A1-treated cells. C. Prolyl 4-hydroxylase (green) and procollagen I (red) in monensin-treated cells. D. Golgi 58K protein (green) and procollagen I (red) in bafilomycin A1-treated cells. Bar, 10 µm.