Hepburne-Scott, Mol Vis 1999; 5:15. Table 1.

Table 1 of Hepburne-Scott, Mol Vis 1999; 5:15.

Table 1. Mutagenic primer sequences

Substitutions were made by incorporating mismatches in the PCR primers. The primer sequences used to investigate each mutation (mutagenic codons are in red).

Mutant F118A

    (Primer 1) 3' - AAGTAGAGGTCCCTCCGGGTGTCCTTCATG - 5'
    (Primer 2) 5' - TTCATCTCCAGGGAGGCCCACAGGAAGTAC - 3'

Mutant K103L/H104I

    (Primer 1) 3' - CTCCAGGTGCCGAACTAGCTTCTTCGCGTC - 5'
    (Primer 2) 5' - GAGGTCCACGGCTTGATCGAAGAAGCGCAG - 3'

Mutant E110H/H111E

    (Primer 1) 3' - CTTGCGGTCCTGGTACTCCCGAAGTAGAGG - 5'
    (Primer 2) 5' - GAACGCCAGGACCATGAGGGCTTCATCTCC - 3'

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