Figure 3 of
Hepburne-Scott, Mol Vis 1999;
5:15.Figure 3 of
Hepburne-Scott, Mol Vis 1999;
5:15.
Figure 3. Chaperone-like activity of wild-type and mutant [alpha]crystallins, using the insulin aggregation assay
Wild-type [alpha]crystallin showed chaperone-like activity at both 1.0 and 0.1 mg concentratons. Each mutant showed identical chaperone-like activity to the wild-type protein at both concentrations.
A. Wild-type [alpha]crystallin. Black line; insulin control, 0.1 mg: Red line; 0.1 mg wild-type [alpha]crystallin: Blue line; 1.0 mg wild-type [alpha]crystallin.
B. Mutant F118A. Black line; insulin control, 0.1 mg: Red line; 0.1 mg mutant F118A: Blue line; 1.0 mg mutant F118A.
C. Mutant K103L/H104I. Black line; insulin control, 0.1 mg: Red line; 0.1 mg mutant K103L/H104I: Blue line; 1.0 mg mutant K103L/H104I.
D. Mutant E110H/H111E. Black line; insulin control, 0.1 mg: Red line; 0.1 mg mutant E110H/H111E: Blue line; 1.0 mg mutant E110H/H111E.
