Figure 1 of
Hepburne-Scott, Mol Vis 1999;
Figure 1. SDS Polyacrylamide gel electrophoresis (SDS PAGE) of expressed mutant [alpha]B crystallins in E. coli
SDS PAGE analysis shows an increase in the level of 20 kDa mutant proteins in post-expression E. coli lysates as compared with pre-expression lysates. Lane 1, marker proteins; lane 2, mutant K103L/H104I, before induction; lane 3, mutant K103L/H104I, after induction; lane 4, mutant F118A, before induction; lane 5, mutant F118A, after induction; lane 6, mutant E110H/H111E, before induction; lane 7, mutant E110H/H111E, after induction; lane 8, bovine [alpha]crystallin. Induction was carried out with 0.4 mM IPTG.