Figure 1 of Hepburne-Scott, Mol Vis 1999; 5:15.

Figure 1. SDS Polyacrylamide gel electrophoresis (SDS PAGE) of expressed mutant [alpha]B crystallins in E. coli

SDS PAGE analysis shows an increase in the level of 20 kDa mutant proteins in post-expression E. coli lysates as compared with pre-expression lysates. Lane 1, marker proteins; lane 2, mutant K103L/H104I, before induction; lane 3, mutant K103L/H104I, after induction; lane 4, mutant F118A, before induction; lane 5, mutant F118A, after induction; lane 6, mutant E110H/H111E, before induction; lane 7, mutant E110H/H111E, after induction; lane 8, bovine [alpha]crystallin. Induction was carried out with 0.4 mM IPTG.

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Hepburne-Scott, Mol Vis 1999; 5:15 <>
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