Figure 2. Sequence of the chicken GCAP1 gene

GCAP1 is encoded by 4 exons (shaded in light blue). Intron sequences are shown in lower case. The residues corresponding to the predicted EF hand Ca²⁺-binding domains are shaded in blue. The translation start (ATG) and stop (TAA) codons are shown in white on navy blue. A possible polyadenylation signal for GCAP1 is boxed in the 3'-UTR. The primers used in the 5'-RACE experiment are indicated in white on black backgrounds. A blue arrow labeled cDNA in the 5'-UTR indicates the 5' extent of the GCAP1 cDNA clones analyzed previously [20]. The blue text interrupted by red residues shows a repeated sequence that is present in the 5'-UTR. The positions of the putative tsp determined by 5'-RACE is indicated with a red arrow. A putative TATA box (+ strand, purple box), putative CAAT box (- strand, green box), Crx-like elements (+ strand, orange boxes), and a CREB/ATF-like element (- strand, blue box) are indicated in the proximal promoter region. The GCAP1 gene sequence is in GenBank as Accession number AF172707.