Figure 8. Purified Sp1 interacts with the overlapping GC and CT boxes

Lanes 2,3,5,7 and 9 incubated with purified human Sp1. Lanes 1-3, WT oligo-186/-148; lanes 4,5, mutant M1; lanes 6,7, mutant M2; lanes 8,9, mutant M3. C: retarded band; ss: supershifted band with Sp1 antibody.

Only the mutation M2, that disrupts the overlapping GC and CT boxes, shown as blue boxes, resulted in complete disappearance of complex C. Disruption of either the GC or CT boxes alone (M1 and M3) does not affect the formation of complex C.