Figure 6. EMSA with double stranded oligonucleotides corresponding to wild type mouse MIP sequence -186/-148 and mutants M1 to M5, incubated with mouse lens nuclear extract.

Lanes 1,3,5,7,9,11, incubation without nuclear extract. Lower panel shows the wild type (WT) -186/-148 sequence and the corresponding mutations introduced in M1 to M5. (*): Mutated nucleotides. Mutant probes are indicated as M1, M2, M3, M4, and M5. The complexes are indicated as C1, C2, and C3. Mutants M2 (lane 6) and M3 (lane 8) do not form complexes C1, C2 and C3. Mutants M1 (lane 4), M4 (lane 10) and M5 (lane 12) form complexes C1, C2 and C3 similar to wild type (lane 2). Only mutations in the CCCCTCCC motif, shown as yellow box, resulted in complete disappearance of the complexes.