Figure 3 of
Kim, Mol Vis 1999;
Figure 3. EMSA with double stranded oligonucleotides corresponding to mouse MIP sequences -186/-124, -90/-36, -128/-69 incubated with mouse lens, [alpha]TN4 (SV), and NIH3T3 (3T3) nuclear extracts.
EMSA was performed with the indicated [32P]-end-labeled probes with 2 µg lens (lanes 2,6,10), [alpha]TN4 (lanes 3,7,11) or NIH3T3 (lanes 4,8,12) nuclear extracts. DNA-protein complexes formed only with lens nuclear extracts are indicated with arrows as C1, C3, and C4. F: free probe. Lower panel shows DNA sequence of probes corresponding to mouse MIP 5'-flanking sequences.