Qian, Mol Vis 4:6, 1998.
Table I. Comparison of Amino Acid Sequences of Skate and Mouse GABA transporters
Percentage Percentage of Similarity of Identity sGAT1 mGAT1 mGAT2 mGAT3 mGAT4 sGAT1 100 89.6 64.7 64.7 65.9 mGAT1 81.2 100 64.2 64.4 65.7 mGAT2 48.7 48.3 100 66.5 63.7 mGAT3 49.8 49.5 78.0 100 78.9 mGAT4 51.3 50.8 74.6 67.6 100
The predicted amino acid sequence of the skate GABA transporter was aligned with mouse GAT-1-4. The numbers in red (left of '100' diagonal) are the percentage of identity calculated by dividing the number of identical amino acids by the total number of amino acids. Numbers in blue (right of '100' diagonal) are the percentages of similarity that include identical amino acids and conserved substitutions.
Table II. Size and junction sequences of introns in the skate GAT1 gene
Intron 5' junction sequence Size, bp 3' junction sequence Position consistent with 1 GAGCCggcaagc ~1.5k tttcaggTGATA 2 GCGGAGgtaaaa ~700 tccaagGGGCAT mouse 3 TCAAAGgtatgg ~700 tcacagGCGTGG human / mouse 4 ACGTCGgtaagt ~300 ttgcagGAACTT human 5 CTGGGAgtaaga 97 ctgcagGCGGAA human / mouse 6 GGAAAGgtaaac ~1.5k ccgcagGTGGTT human / mouse 7 TCCGAGgtaggt 127 tctcagGTATGG human 8 ATACAGgtaaca ~2.3k gttcagAGACTC human / mouse 9 CTTCAGgtatga ~2.3k ttcaagGTCCTG human / mouse 10 AGCCAGgtaagc ~4.5k tttcagTTTTGC human / mouse 11 ACCCAGgtaaat ~10k ccctagGGTGGT human / mouse 12 GCTATGgttagt ~10k ctccagGTGTGA human 13 GTGGGGgtaaga ~700 cctcagGGTGTG human 14 AAACAGgtagtg ~4.5k gttgagCGTCTG human / mouse
The introns in the SGAT-1 gene were obtained by PCR from the skate genomic DNA and the intron positions were determined by sequencing. The exon sequence is shown in the upper case and the intron sequence is shown in the lower case. The intron positions in the SGAT-1 coding region were compared with that in the human and mouse GAT-1 genes as shown in the rightmost columns.