Table 1 of
Baer, Mol Vis 4:30, 1998.Table 1 of
Baer, Mol Vis 4:30, 1998.
Table 1. Summary of binding parameters determined by fluorescence enhancement titrations
The binding of 9-(9-anthroyloxy) stearic acid (9-AS) and all-trans retinol to X4IRBP and the Arg->Gln substitution mutants was followed by monitoring ligand-fluorescence enhancement. The number of ligand-binding sites (N) and the dissociation constant (Kd) were determined as described by Baer et al. [48].
Protein 9-AS Enhancement* All-trans retinol Enhancement#
------------ -------------------------- ------------------------------
N Kd N Kd
--------- -------------- --------- ------------
X4IRBP 1.88±0.06 0.065±0.029 µM 1.28±0.19 0.72±0.18 µM
Arg1005->Gln 4.27±0.09 0.16 ±0.06 µM 2.95±0.15 0.80±0.11 µM
Arg1041->Gln 2.10±0.17 0.19 ±0.11 µM 0.76±0.30 1.36±0.31 µM
Arg1073->Gln 2.59±0.09 0.083±0.047 µM 1.38±0.30 0.92±0.25 µM
Arg1122->Gln 2.18±0.06 0.11 ±0.04 µM 1.15±0.21 1.36±0.26 µM
* Monitored at 440 nm upon excitation at 360 nm.
# Monitored at 480 nm upon excitation at 330 nm.
|