Figure 7 of
Baer, Mol Vis 4:30, 1998.
Figure 7. Normalized 9-AS fluorescence enhancement titrations comparing X4IRBP and the Arg1005->Gln mutant
The binding of 9-AS to 1.33 µM of X4IRBP (filled symbols) and 0.92 µM of the Arg1005->Gln mutant (open symbols) was monitored at 440 nm upon excitation at 360 nm. For X4IRBP, N = 0.87±0.12 and Kd = 0.51±0.09 µM. For Arg1005->Gln, N = 5.00±0.29 and Kd = 0.05±0.07 µM. Nonnormalized data is shown as an insert.