Figure 6 of
Baer, Mol Vis 4:30, 1998.Figure 6 of
Baer, Mol Vis 4:30, 1998.
Figure 6. Normalized association curves of 9-AS with X4IRBP and the Arg1005->Gln mutant monitored by fluorescence over time
The association curve of X4IRBP (filled symbols) shows the increase in absolute fluorescence after addition of 0.92 µM 9-AS to 1.3 µM X4IRBP. The association curve of Arg1005->Gln (open symbols) shows the increase in absolute fluorescence after the addition of 0.96 µM 9-AS to 0.92 µM Arg1005->Gln. In both cases fluorescence was monitored at 440 nm upon excitation at 360 nm. The half-time for association increased from 1.24 min for X4IRBP to 4.19 min for the Arg1005->Gln mutant.
