Figure 2 of
Baer, Mol Vis 4:30, 1998.
Figure 2. Creation of Arg->Gln substitution mutants by overlap extension mutagenesis
(A) The table provides the sense primers containing the mutated codon (underlined). (B) Diagram of the overlap PCR mutagenesis strategy (see Methods). (C) The mutants have been confirmed by automated fluorescence DNA sequencing. The sequences shown correspond to the non-coding strand.