Figure 13. Protection of all-trans retinol by X4IRBP and the four Arg->Gln mutants

All-trans retinol was added to a solution of 2.7 µM recombinant protein to a final concentration of 3.2 µM. The absorbance of all-trans retinol was monitored at 325 nm every two min for 2 h. The ethanol concentration was 0.74%.