Figure 10 of
Baer, Mol Vis 4:30, 1998.
Figure 10. Normalized all-trans retinol fluorescence enhancement titrations showing direct comparison between X4IRBP and the Arg1005->Gln mutant
The binding of all-trans retinol to 1.33 µM of X4IRBP (filled symbols) and 0.92 µM of the Arg1005->Gln mutant (open symbols) was monitored at 480 nm upon excitation at 330 nm. For X4IRBP, N = 1.50±0.31 and Kd = 0.80±0.26 µM. For Arg1005->Gln, N = 3.67±0.49 and Kd = 0.93±0.26 µM. Nonnormalized data is shown as an insert.