Figure 2 of
Weiss, Mol Vis 4:27, 1998.Figure 2 of
Weiss, Mol Vis 4:27, 1998.
Figure 2. Comparison of the amino-acid sequence of ground squirrel GRK7 to GRK7 from Oryzias latipes and bovine GRK1.
The hyphens (-) represent sequence in GRK7 from Oryzias latipes (Ol) and bovine (Bv) GRK1 that is identical with the sequence of ground squirrel (GS) GRK7. Gaps in the sequence alignment are represented by periods (.) The blue lettered region represents the predicted catalytic domain (amino acids 185-464 of the ground squirrel sequence). The asterisks (*) mark serine and threonine residues that serve as known or potential autophosphorylation sites. The sequence with the double underline represents the CAAX motif, which codes for isoprenylation at the cysteine residue. Sequence comparisons were performed using the computer programs PILEUP and PRETTY from the Wisconsin Package Version 9.0. The nucleotide sequence for 13-lined ground squirrel GRK7 has been deposited in the GenBank database under the GenBank Accession Number AF063016.
GRK7 (GS) M.DMGGLDNL IANTAYLQAR KT.DSDSREL QRRRRSLA.. .LPGPQGCAE 45
GRK7 (Ol) -C-------- V------K-Q ...GG-DK-M KK-----S.. .--K-EQ-VA 44
GRK1 (Bv) -.-F-S-ETV V--S-FIA-- GSF-AS-GPA S-D-KY--RL K--PLSK-EA 49
*
GRK7 (GS) LRQSLSPHFH SLCEQQPIGR RLFRDFLATV PKYSQAVAFL EDVQNWELAE 95
GRK7 (Ol) --E-IEKD-T L---R----K --------NT -EFKL-AEF- DELYD-D--- 94
GRK1 (Bv) --E--DLG-E GM-LE----K ---QQ--R-H EQHGP-LQLW K-IEDYDT-D 99
GRK7 (GS) EGPAKTSTLQ QLAATCARDP GP.QSFLSQD LATKCRAAST DEERKTLVEQ 144
GRK7 (Ol) GAAKDKARQN IINKY-KP-S KTFLT---GE P-E--KSVTD ATFEEVMKNK 144
GRK1 (Bv) DALRPQKAQA LR--YLEPQA QLFC---DAE TVARA--GAG -GLFQP-LR. 148
GRK7 (GS) AKAETMSFLQ EQPFQDFLAS PFYDRFLQWK LFEMQPVSDK YFTEFRVLGK 194
GRK7 (Ol) VQDGVRE--K GK--TEYQG- QYF-K----- EY-K--I--- --Y---T--- 194
GRK1 (Bv) ...AVLAH-G QA---E--D- LYFL------ WL-A--MGED W-LD--V--R 195
GRK7 (GS) GGFGEVCAVQ VRNTGKMYAC KKLDKKRLKK KGGEKMALLE KEILEKVNSP 244
GRK7 (Ol) ---------- -K---Q---- ---C------ ---------- -Q-------L 244
GRK1 (Bv) ------F-C- MKA---L--- ---N------ RK-YQG-MV- -K--A--H-R 245
GRK7 (GS) FIVSLAYAFE SKTHLCLVMS LMNGGDLKFH IYNVG..... .TRGLAMSRV 288
GRK7 (Ol) -L-N----YD T--------T --------Y- ---I-YDGKG VDK-IE-K-I 294
GRK1 (Bv) ---------- T--D-----T I-----IRY- ----DEDNP. ...-FQEP-A 291
GRK7 (GS) IFYTAQMTCG VLHLHGLGIV YRDLKPENVL LDDLGNCRLS DLGLAVEVQD 338
GRK7 (Ol) -H----I-T- I----DMD-I ---M------ --SQ-Q---- -----I-IAP 344
GRK1 (Bv) ------IVS- LE---QRN-I ---------- ---D--V-I- -------LKA 341
GRK7 (GS) DKPITQ.RAG TNGYMAPEIL MDKASYSYPV DWFAMGCSIY EMVAGRTPFK 387
GRK7 (Ol) G-TV--.M-- -GA------- .S-TP-RTS- --W-L----- -----Y---- 392
GRK1 (Bv) GQTK-KGY-- -P-F----L- LGE.E-DFS- -Y--L-VTL- --I-A-G--R 390
GRK7 (GS) ...DFKEKVS KEDLKERTMK DEVAFHHENF TEETKDICRL FLAKKPEQRL 434
GRK7 (Ol) GPESK----E --EVQR-ILN E-PKWE-KC- DAP---VIQQ --K--IDE-- 442
GRK1 (Bv) ARG...---E NKE--Q-VLE QA-TYPDK.- SPAS--F-EA L-Q-D--K-- 436
GRK7 (GS) GSRE.KADDP RKHPFFQTVN FPRLEAGLVE PPFVPDPSVV YAKDVDEIDD 483
GRK7 (Ol) -M-N.NME-- ---EW-KSI- ---------D --W--K-N-- ----TGD-AE 491
GRK1 (Bv) -F-DGSC-GL -T--L-RDIS WRQ----MLT ------SRT- ---NIQDVGA 486
GRK7 (GS) FSEVRGVEFD DKDKQFFQRF STGAVPVAWQ EEIIETGLFE ELN....... 526
GRK7 (Ol) ---IK-I--- A--DK--KE- ------IQ-- Q-M----L-D ---....... 534
GRK1 (Bv) --T-K--A-E KA-TE---E- AS-TC-IP-- --M----V-G D--VWRPDGQ 536
**
GRK7 (GS) ..DPNRPSGD GKG.DSSKSG VCLLL 548
GRK7 (Ol) ..----KE-A -G-D-EK--- T-A-- 557
GRK1 (Bv) MP-DMKGVSG QEAAP----- M-V-S 561
====
|