Figure 6 of
Stenkamp, Mol Vis 4:26, 1998.
Figure 6. IRBP immunoreactivity in the developing and adult zebrafish eye
(A) 65 hours post-fertilization (hpf) eye. Specific IRBP immunoreactivity is not present in the developing retina or RPE. L, lens. (B) 65 hpf eye. The signal around the developing lens (L) is also seen in eyes reacted with rabbit pre-immune serum. (C) Confocal micrograph of a 72 hpf eye. IRBP immunoreactivity is detectable in a few cells of the photoreceptor layer (arrows). (D) Confocal micrograph of a 77 hpf eye; view of the most mature portion of the retina, near the optic disk. Immunoreactivity is localized to the apical portion of developing photoreceptors (arrow). E: 79 hpf, IRBP immunoreactivity is detectable across the entire zebrafish retina (arrows). F: Adult retina at mid-light. IRBP is localized to the region between the outer limiting membrane (OLM) and the RPE. IRBP appears to associate with the outer segments (OS) of cone photoreceptors (arrow). Asterisks (*) in each panel indicate location of RPE/choroid. Scale bar is 50 µm in Panels A,B,C,E; 20 µm in D, F. Nuclear stain is 4',6-diamidino-2-phenylindole (DAPI). ONL, outer nuclear layer.