Figure 3 of
Bernstein, Mol Vis 1998;
Figure 3. Northern analysis of total RNA using cDNA probes from photoreceptor structural genes
Total RNA (5 mg) from each of pooled human fovea (HF), pooled human midperipheral retina (HP), pooled monkey fovea (MF), and pooled monkey midperipheral retina (MP) were denatured, electrophoresed, transferred to nylon membranes and reacted with the radiolabelled cDNA probes as described in methods. Following hybridization, the blots were stringently washed (63 °C/0.2X SSC), and exposed to X-ray film at -70 °C for varying lengths of time. Shown below each autoradiograph are the ethidium bromide stained 18S ribosomal RNA bands from the original agarose gel from which the analyzed blot was made. The ethidium bromide stained 18S rRNA band was analyzed densitometrically and used as an internal RNA loading standard.
Figure 3A. RDS/peripherin, 5 day exposure
Figure 3B. ROM-1, 5 day exposure