Figure 2 of
Bernstein, Mol Vis 1998;
Figure 2. Northern analysis of total RNA using cDNA probes from photoreceptor pigment genes
Total RNA (5 mg) from each of pooled human fovea (HF), pooled human midperipheral retina (HPR), pooled monkey fovea (MF), and pooled monkey midperipheral retina (MPR) were denatured, electrophoresed, transferred to nylon membranes and reacted with the radiolabelled cDNA probes as described in the methods section. Following hybridization, the blots were stringently washed (63 °C/0.2X SSC), and exposed to X-ray film at -70 °C for varying lengths of time. Shown below each autoradiograph are the ethidium bromide stained 18S ribosomal RNA bands from the original agarose gel from which the analyzed blot was made. The ethidium bromide stained 18S rRNA bands were densitometrically analyzed and used as internal RNA loading standards, to normalize for sample variations in total RNA loading.
Figure 2A. Red/green photoreceptor pigment, 48 hr exposure
Figure 2B. Human opsin, 72 hr exposure
Figure 2C. The a-subunit of rod phosphodiesterase, 48 hr exposure