Figure 9. Association of vinculin, smooth muscle [alpha]-actin and [alpha]-actinin with PI3-kinase in the cytoskeleton

Cytoskeleton fractions of the first passage CECs and modulated CECs were prepared, and proteins (30 µg/sample) associated with PI3-kinase were detected by immunoprecipitation with PI3-kinase antibody and immunoblotting with vinculin antibody, smooth muscle [alpha]-actin antibody or [alpha]-actinin antibody. 1, normal CECs; 2, CECs modulated with FGF-2. M, protein size marker.